Proteins and peptides

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Overview

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

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Postnova EAF2000 electrical asymmetrical flow FFF system
The Postnova EAF2000 system is based on the AF2000 asymmetrical flow FFF module, with an additional Electrical FFF module, a special EAF4 channel and a software module. The EAF2000 is ideal for many applications in the domains of biopharm, environment and nanomaterials.

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Postnova AF2000 MT mid temperature MultiFlow FFF system
The system can be configured for Asymmetric Flow Field-Flow Factionation (AF4), Hollow Fiber Flow Field-Flow Factionation (HF5) and Size Exclusion Chromatography (SEC).

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Postnova CF2000 Centrifugal FFF system
The system can separate and distinguish relative size differences of 5% only and can be used to monitor absolute size differences down to 1 nm. These unique features make it the ideal choice for any nanoparticle research and QC tasks.

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Postnova 21-angle MALS detector - the ideal detector for ultra-high molar mass
The Postnova 21-angle MALS detector has been especially developed for coupling with FFF systems – to the opposite of previously available MALS detectors which were designed with chromatography (SEC/GPC) in mind, that is for smaller molar mass.

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High sensitivity online Dynamic Light Scattering detector, special customized for use connected to FFF
Online DLS measurements using a special flow cell can be perfomed to obtain particle size (Rh) and molecular weight (Mw) distributions of particles and molecules separated by Postnova Asymmetric Flow FFF (AF4), Centrifugal FFF (CF3) and Thermal FFF (TF3) systems.

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Mapping analysis of secondary structure of protein using microscope FTIR
The distribution maps of secondary structures of proteins, based on the data of the mapping measurement using microscope FTIR, are shown in this application data.

Measurement of protein in heavy water by FT-IR
This application note demonstrates FT/IR measurements of several types of protein in heavy water.

CD measurement of protein film samples in the vacuum UV region
Marker bands for specific secondary structures are most typically observed in the UV region below 260 nm and as far into the far-UV region as possible. It has been widely reported that CD measurements using synchroton radiation have been used to successfully measure spectra down to around 140 nm. As an alternative to synchrotron radiation, the J-1500 CD spectrometer has been improved for measurements in the vacuum UV region.

Application notes

Electrical asymmetrical flow field-flow fractionation analysis of the NIST monoclonal antibody reference material 8671
The electrical asymmetrical flow field-flow fractionation system allows both size and surface charge separation, enabling measurement of size or molecular weight distribution and electrophoretic mobility.