Measurement of protein film samples in vacuum ultraviolet region by using Jasco J-1500 CD spectrometer

Measurement of protein film samples in vacuum ultraviolet region by using J-1500 CD spectrometer

CD spectroscopy is considered as an essential tool to analyze the structure of protein samples. CD spectra reflecting the secondary structure of protein are usually observed from UV region below 260 nm to the vacuum ultraviolet region. Nowadays, it has been reported as an example that by CD instrument utilizing synchrotron radiation the protein film sample was successfully measured down to around 140 nm. CD analysis by using synchrotron radiation is a very effective method to measure CD in vacuum ultraviolet region, however, it is not easy since it requires a very special facility. JASCO Model J-1500, having the most updated electric system using the latest digital lock-in detection capability, the further improved optical system with higher light through-put and N2 purge function with higher efficiency which was developed based on flow simulation technology, enables to achieve high S/N CD measurement even in vacuum ultraviolet region. This note shows the analysis example of protein film sample in vacuum ultraviolet region by using J-1500 CD spectrometer.