Relative quantum yield measurement using Jasco FP-8000 series spectrofluorometers

Fluorescence quantum yield is defined as the ratio of the number of photons emitted from sample as fluorescence to the number of photons in the excited light absorbed. Absolute method and relative method are known measuring methods. Regarding the absolute method, it is necessary to detect all the fluorescence from the sample, requiring the integrating sphere, while relative method can calculate quantum yield of unknown sample by comparing the intensity of standard fluorescence with unknown sample, and accordingly the relative method is easier to get the results of quantum yield.

In this experiment, an example will be shown for the calculation of quantum yield of Rhodamine B when fluorescein is used as the standard sample.