Absolute quantum yield measurement of solution using Jasco FP-8000 series spectrofluorometers

Fluorescence quantum yield is defined as the ratio of the number of photons emitted from sample as fluorescence to the number of photons in the excited light absorbed. Absolute method and relative method are known as measuring methods. Relative method is comparing the intensity of standard fluorescence with unknown sample to calculate quantum yield of the unknown sample. Therefore obtained results depend on the accuracy of standard sample’s quantum yield value. On the other hand, quantum yield can be obtained directly by the absolute method, because the absolute method allows to detect all the fluorescence from the sample and integrates using integrating sphere, enabling more accurate quantum yield measurement.

In this experiment, some examples will be shown for the calculation of solution sample’s quantum yield of which published values from literature are known by the absolute method.