Analysis of aldehydes using post-column derivatization by High Performance Liquid Chromatography

It is becoming a big concern that aldehydes such as the formaldehyde and acetaldehyde as an environmental pollutant may contaminate environment such as the atmosphere, lakes and marshes, reservoirs, and rivers. Therefore, it is an object of various regulations like Air Pollution Control Law, Water Supply Law, and Offensive Odor Control Law, etc. in this country. As a method to measure aldehydes using HPLC, the pre-column derivatization method by 2,4- DNPH is well known, while the pretreatment such as sample collection, condensation and extraction is necessary. JASCO has introduced so far the analysis of formaldehyde and acetaldehyde by the post column fluorescence derivatization method using 1,3- Cyclohexanedione as a derivatizing reagent, which doesn't need such pretreatment like condensation etc.

Here, in addition to two components, five components including propyl aldehyde, butyraldehyde, and valeraldehyde were analyzed simultaneously.