Measurement of the complex forming reaction of nickel sulfate and Rochelle salt probed by stopped-flow CD spectroscopy

Stopped-flow CD is a well-known method to probe the unfolding and refolding processes of proteins in order to obtain secondary structure information on a millisecond time scale. Stopped-flow CD can also be used outside the realm of protein dynamics, to observe complex-forming reactions. Transition metal complexes typically exhibit absorption bands in the visible to near-infrared region of the spectrum and CD measurements can be used to probe structural changes occurring in this region.

This application note demonstrates the measurement of the complex-forming reaction of nickel sulfate and Rochelle salt using a High Speed Stopped-Flow system consisting of a J-1500 CD spectrometer and a SFS-562T stopped-flow accessory.