Measurement of NIR-CD spectra of nickel titrate and limonene using a J-1500 CD spectrometer

In the near-infrared (NIR) region, observed CD spectra are a result of the combination and overtone vibrational modes of the O-H and C-H transitions (NIR-VCD), as well as the d-d transition of metallo-proteins and metal complexes (NIR-ECD). NIR-ECD spectra of metallo-protein and metal complexes are known to be sensitive to conformations of the metals ligands as well as the configuration around the central metal atom, which is used for structural analysis of these molecules. For NIR-VCD, theories and calculation methods to interpret the NIR-VCD spectra have been introduced and comparative analysis for many of the spectra have been accomplished.

The J-1500 CD spectrometer can be used for spectral measurements into the NIR region (up to 1600 nm) using an extended wavelength range light source and detector. In this configuration, the instrument can be used for NIRVCD measurements of vibrational transitions and NIR-ECD measurements of metal complexes. The J-1500 can also be used to measure the ECD spectrum in the UV region, primarily for the study of biological samples.

This application notes demonstrates the use of the J-1500 CD spectrometer to probe vibrational and electronic transitions of nickel tartrate solution and limonene in the NIR region of the spectrum.