Measurement of the reduction reaction of 2,6-dichloroindophenol (DCIP) using the absorption stopped-flow system

Using the absorption stopped-flow measurement system, consisting of FS-110 fast scan spectrophotometer and SFS-852 stopped-flow system, two to four kinds of liquid sample can be mixed quickly and the changes in absorption spectra can be measured at intervals of 5 msec. This system allows measurement of rapid enzymatic, catalytic and oxidation-reduction reactions.

This application note illustrates an example of determining the reaction rate by using the absorption stopped-flow system for the reduction of DCIP, whose color in aqueous solution is changed from blue to colorless as a result of reaction with L-ascorbic acid.