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Analysis of free fatty acids in sardine oil using HPLC

Resource type: 
Application note
Author(s): 
Jasco
Format: 
pdf
Date of creation: 
18 June 2017
Library code: 
10979

It is well-known that a lot of beneficial bioactive substances are included in sardine oil. The free fatty acids in sardine oil were analyzed by using pre-column derivatization method with p-bromophenacyl bromide in order to detect them with high sensitivity and selectivity. A silica C18 column Finepak SIL C18S was employed for the separation. A solution of acetonitrile and water was used for as mobile phase in gradient elution method.