Analysis of amino acids using pre-column derivatization with DABS-Cl
Resource type:
Application note
Format:
pdf
Date of creation:
24 June 2017
Library code:
10990
A small amount of amino acids of proteins and peptides can be quantitatively analyzed by vapor-phase hydrolyzing with hydrochloric acid, derivatizing with DABS-Cl (Dimethylamino azobenzene-sulfonyl chloride) and separated on reversed phase HPLC.
DAB-Label is a kit to facilitate the troublesome work for derivatization. The obtained DAB-Labeled amino acids provide good stability and can be stored for quite a while.
