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Amino acid composition analysis of protein using pre-column derivatization with DABS-Cl

Resource type:

Application note

Author(s):

Jasco

Format:

pdf

Date of creation:

24 June 2017

Library code:

10991

Amino acid composition of protein was analyzed by DAB-Label which is a kit for pre-column derivatization amino acid analysis with DABS-Cl (Dimethylamino azobenzene-sulfonyl chloride).

1 ug of Myoglobin was vapor-phase hydrolyzed with hydrochloric acid (6N HCl, 110 ̊C, 24 hrs) and derivatized with DABS-Cl. The analysis of the derivatives was performed by HPLC.

Download Application note, Amino acid composition analysis of protein using pre-column derivatization with DABS-Cl.pdf (155.68 KB)