Analysis of chondroitin sulfate sodium salt by SEC

There are five kinds of chondroitin sulfate (MW 20,000 ~ 50,000) which is one of mucopolysaccharide, such as A~E, contained in connective tissues of animal’s cartilagines and skin, and they are used for drug medicines and healthy food. By using a column with exclusion limit of 300,000 molecular weight (Pullulan) for SEC (Size Exclusion Chromatography), chondroitin sulfate sodium salt was analyzed with an RI detector. The calibration curve was created with Pullulan as standard mixture by ChromNAV GPC Calculation Program (Optional) and the result calculated based on Pullulan converted molecular weight is shown in this application note.