High-speed analysis of Dabsyl amino acids in beer by UHPLC

There are several methods for amino acids analysis, such as separation by reversed-phase column after pre-column derivatization and detection using a UV/Vis detector, or post-column derivatization after separation by ion-exchange column and detection using a fluorescence detector. Among them, pre-column derivatization method using Dabsyl chloride as reagent is well known, because Dabsyl chloride reacts easily with both of primary and secondary amino acids, and derivatized amino acids are stable, assuring easy handling, good accuracy and high sensitivity. In order to enable the easier derivatization method, DAB-Label kit is available from Jasco.
Here, the amino acids in stout beer were measured using DAB-Label derivatization kit by Ultra High-performance Liquid Chromatography (UHPLC) with a UV-Vis detector.