High resolution analysis of tryptic digests of bovine serum albumin by UHPLC with photodiode array detection

The peptide mapping is one of the testing methods for biomedicine. This method requires HPLC or other separation analysis of peptide segments digested by enzymes or chemicals, and it is one of the internationally harmonized methods among the U.S. Pharmacopoeia (USP), the European Pharmacopoeia (EP) and the Japanese Pharmacopoeia (JP). In this method, the standard protein is digested in the same manner as in the target protein in order to directly compare the peptide mapping of standard protein with that of the target protein so that the expression of recombinant proteins can be detected. The method is applied to quality assurance of various food products.

In this report, the peptide mapping of tryptic digests of BSA using the X-LC system is demonstrated.