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Ultra-sensitive method for the determination of 1,25 di-OH D2 and D3 vitamins in serum using supported liquid extraction prior to LC-MS/MS

Resource type: 
Application note
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
1 August 2017
Library code: 
11160

This application note describes a protocol for the extraction of 1,25 di-OH vitamin D2 and 1,25 di-OH vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. The method described in this application achieves highly reproducible recoveries for 1,25 diOH vitamins D2 and D3. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

PDF icon Download Application note, Ultra-sensitive method for the determination of 1,25 di-OH D2 and D3 vitamins in serum using supported liquid extraction prior to LC-MS-MS.pdf (1.77 MB)