Extraction of methylmalonic acid from serum using ISOLUTE PLD+ prior to LC-MS-MS analysis
Resource type:
Application note
Format:
pdf
Date of creation:
1 August 2017
Library code:
11162
ISOLUTE PLD+ protein and phospholipid removal products offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation. Requiring next to no method development, ISOLUTE PLD+ can be integrated quickly and easily into routine workflow, increasing productivity and reducing instrument downtime.
This application note describes a simple, effective ISOLUTE PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts.
