Extraction of acrylamide from coffee using ISOLUTE SLE+ prior to LC-MS/MS analysis

ISOLUTE SLE+ products provide clean, rapid, robust and efficient extraction solutions for a wide range of analytes.

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE SLE+ columns with LC-MS/MS detection. The method described achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppb (25 ng/g) in ground coffee (solid) or 125 ppb (125 ng/g) in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.