Flash chromatography

Flash purification systems, consumables and accessories

Biotage, pioneers of flash chromatography, have a proven record of innovation that began twenty years ago with the development of the first packed flash chromatography columns and first microprocessor controlled purification systems. This strong tradition continues today with Accelerated Chromatographic Isolation (ACI), a revolutionary approach which significantly improves the quality and efficiency of the process, taking flash to a whole new level. Biotage’s extensive range of instrumentation is complemented by a complete range of flash consumables.

Here are a few of the truly unique flash purification solutions that Biotage offers:

Compound identification by mass during purification

Isolera Dalton brings mass detection to flash chromatography. This system identifies compounds by mass in real time during flash separation, leading to greater confidence in purification and a significant saving in time and money. This combination of identification and purification removes complex off-line analytical steps from the workflow, vastly increasing throughput and putting the entire purification and analysis in the hands of the chemist. Reactor content can be injected directly to identify target masses, which then are transferred directly to the Flash method for mass targeted purification.




Isolera Dalton Mass Directed Flash Chromatography

Accelerated Chromatographic Isolation (ACI)

Taking flash purification to another level Biotage has developed ACI, a revolutionary advancement converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis.




Isolera Spektra System with ACI

The solution for hard-to-dissolve crude samples

One of the most common flash purification challenges is dealing with hard-to-dissolve crude samples. Because polar solvents cause poor chromatographic results when used as injection solvents in normal-phase flash chromatography, other sample load options are needed. A commonly used option is dry loading, which involves dissolving the sample in a suitable solvent, mixing with an inert, clean, dry adsorbent, such as silica, alumina, or diatomaceous earth, and drying the slurry. The dried sample is then loaded into an empty vessel and inserted ahead of the purification cartridge. By drying the mixture, the polar solvent is removed and will have no impact on the purification. Dry load can be usually achieved within the flash system but sometimes the amount of adsorbent needed exceed the internal capacity. When that situation arises, the Biotage external Dry Load Vessels (DLV), with capacity up to 500 g),  provide a solution.




A DLV-500 mounted on an Isolera LS system