An automated dual stage SPE procedure for 15 F2t-isoprostane (8-iso prostaglandin2a) from BSA as lipid markers of oxidative stress

F2-isoprostanes are lipid markers of oxidative stress in animal host systems. F2-isoprostanes have been identified as biomarkers of cardiovascular disease, pulmonary disease, neurological disorders and various conditions related to the health and function of the kidneys and liver. Strategies to measure these analytes are typically laborious multistep procedures that are plauged with low recovery sample preparation strategies leading to poor method precision. Targeting endogenous lipids as analytes of interest is challenging since modern solid phase extraction procedures (SPE) typically aim to remove lipids in favor of targeting small molecules. For these reasons, method optimization strategies are required to improve the efficacy of current testing protocols.

In this work, the automation of an exisiting SPE procedure was implemented. A dual stage cleanup strategy was employed. It is anticpated that this report will have impact in labs that provide testing platforms for population studies focused on lipids as markers of oxidative stress. This automation platform demonstrates an improvement in lab efficiency and data quality.