Evaluation of 25-hydroxy vitamin D extraction using phospholipid depletion plate technology and method comparison using automated sample preparation

Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems and is associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. Many sample preparation approaches to the extraction of 25-hydroxy vitamin D have been employed prior to LC-MS/MS analysis. Simple protein precipitation, supported liquid extraction and complex SPE methodology are all in routine use.

This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared.