Extraction of catecholamine acid metabolites from plasma prior to analysis using UHPLC-MS/MS
Resource type:
Poster
Format:
pdf
Date of creation:
12 February 2018
Library code:
11602
Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5 HIAA) are biomarkers for neuroblastoma and catecholamine-secreting tumors. This poster presents optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Parameters investigated include MRM transitions, chromatography and sample preparation protocols.
