Effective extraction strategies for buprenorphine and norbuprenorphine in urine, oral fluid and whole blood using cation exchange solid phase extraction and supported liquid extraction prior to HPLC-MS/MS analysis

Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. A fast, reliable and robust sample preparation method that could be implemented to extract these drugs from complex biological matrices with good analyte recovery and minimum matrix effects would be ideal for toxicology labs.

This poster demonstrates two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine, oral fluid and whole blood.