Highly efficient CD/CPL systems for accurate simultaneous CD/Abs and CPL/FL measurements by Jasco

CD and CPL spectrometers are widely used for evaluation of the structure of chiral compounds, secondary structure estimation (SSE) for proteins and CPL characterization of synthetic compounds. Most CD applications require not only the CD spectrum, but also the absorption (Abs) spectrum obtained using a UV/Vis spectrophotometer. For phosphors with CPL properties, the fluorescence (FL) spectrum of the sample is recorded using a spectrofluorometer and the CPL and DC spectra are measured using a CPL spectrometer to obtain the glum value.

Here, Jasco describes example measurements using a J-1500 CD spectrometer, which can simultaneously record highly accurate CD and Abs spectra. The method for obtaining the FL spectrum using a CPL-300 CPL spectrometer and the accuracy of the spectral shape are discussed. An example of simultaneous measurement of CPL and FL spectra with the correct spectral shape for fluorescent substances such as Eu (facam)3 and camphor is presented.