Biotage Extrahera precision and accuracy performance part 1
Resource type:
Technical note
Format:
pdf
Date of creation:
3 December 2015
Library code:
10483
This document details an example of the pipetting precision and accuracy data for four specified volumes across a full 96 well plate.
Experimental design
- Precision and accuracy were tested by separately measuring the volume of 96 transfers of 50 μL, 100 μL, 500 μL and 1000 μL
- Water was dispensed sequentially (8 channels simultaneously) starting with column 1 (positions A1-H1) and ending with column 12 (positions A12-H12)
- Performance was assessed by measuring the weights of 96 collection tubes before and after the addition of purified water
- Volume data was generated for each position using temperature corrected density values
Because of the low volumes and weights involved, correction factors for temperature and evaporation rate were applied:
