CD thermal denaturation measurement of minute sample volumes

Circular Dichroism (CD) measurement is an effective tool for secondary structure analysis and the thermal denaturation analysis of proteins and nucleic acids. A rectangular cell of 1 mm optical path length is generally used for Far UV measurement, requiring approximately 200 uL of sample volume. There has been great interest in an apparatus and technique to carry out CD measurements on very low volumes in the case of precious samples where only a tiny amount can be purified.

Jasco now offers a new capillary cell and capillary jacket for thermal ramping analysis of sample volumes less than 10 uL. Measurement is simple and the capillary cells are inexpensive and disposable.