ISOLUTE SLE+ supported liquid extraction plates for the extraction of drugs from biological fluids

Liquid-liquid extraction (LLE) is a commonly used sample preparation technique for the isolation of drugs from biological fluids. While the technique provides clean extracts, time consuming off-line steps such as capping, mixing, shaking and de-capping, along with possible emulsion formation do not make the technique amenable to high throughput sample preparation.

Supported Liquid Extraction (SLE) is a high throughput alternative to LLE. The technique uses an inert support to mimic the LLE process and does not have many of the problems associated with the traditional technique. ISOLUTE SLE+ 200 and 400 mg supported liquid extraction plates contain a modified form of diatomaceous earth. The support provides an inert interface between the two liquids, allowing rapid transfer of the drugs into the extraction solvent.

This technical note introduces Biotage's ISOLUTE SLE+ supported liquid extraction plates, it contains useful information and examples of uses for them.