Asymmetric flow FFF (AF4), analytical ultracentrifugation and size exclusion chromatography monoclonal antibody aggregate quantification
Resource type:
Technical note
Format:
pdf
Date of creation:
22 August 2018
Library code:
12181
AF4 is a separation technique using an unpacked separation channel instead of a packed column. Lack of a stationary phase provides unique advantages, which are: no shear forces, matrix interaction-free sample separation, fast separation times and great flexibility with respect to the choice of solvent. Also, AF4 offers a wide separation range from 1 kDa/ 1 nm up to several MDa/ 10 um. Thus, a complete picture of the aggregation phenomena is provided, including monomer, soluble and insoluble aggregates and even the subvisible particle fraction.
