Extraction of urinary hormone metabolites using supported liquid extraction prior to HPLC-MS/MS analysis

In this poster, Biotage demonstrates a rapid and reliable sample preparation method using support liquid extraction (SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.