Norlab

Your partner in Northern Europe for innovative laboratory instruments

Supported liquid extraction of vitamin D metabolites: 25-hydroxy and 1α, 25-dihydroxy vitamin D2/D3 using PTAD derivatization prior to LC-MS/MS analysis

Resource type: 
Poster
Author(s): 
Biotage
Format: 
pdf
Date of creation: 
2 March 2018
Library code: 
11621

In this poster, high analyte recoveries and low ion suppression are demonstrated, allowing limits of quantitation at low pg/mL levels for the di-OH metabolites and < 1 ng/mL levels for the OH metabolites. The method demonstrates good linearity and coefficients of determination from calibration curves.