Using a semi-preparative reversed phase HPLC column on Biotage Selekt
Resource type:
Technical note
Format:
pdf
Date of creation:
7 November 2019
Library code:
12725
The Biotage Selekt flash chromatography instrument can be run at a maximum flowrate of 300 mL/min or a maximum pressure of 30 bar. These high flowrates and pressures enable a user to perform chromatography using not only dry-packed, single-use plastic flash columns containing small (≥20 um) spherical silica particles, but also semi-preparative, slurry-packed HPLC columns for multiple use with smaller (≤20 um) spherical silica particles.
In this note, Biotage shows the separation of a four-component mixture using a semi-preparative, slurry-packed reversed phase HPLC column with 15 um spherical silica particles and a Biotage Selekt instrument.
