CPL spectrum measurement of green fluorescent protein

CPL spectrum measurement of green fluorescent protein

Circularly Polarized Luminescence (CPL) is the difference of circular polarized fluorescence intensity between right-handed and left-handed which is emitted from chiral compounds under exited condition. There are several main target samples and applications in CPL measurement as fluorescent chiral organic compound and chiral lanthanoid complex. And biomacromolecule such as Green Fluorescent Protein (GFP) is also different target sample. GFP is a fluorescent protein which isolated from Aequorea victoria, the CPL spectra of the protein have already been reported, which indicates that living jelly fish may emit the circular polarized light. And Mantis shrimp is known to perceive circular polarized light and this detail mechanism is expected to be elucidated in future related with the research progress. This application note shows CPL measurement results about GFP fluorescence protein by using CPL-300.