CD measurement at NIR region by J-1500

Measurement of nickel tartrate and limonene

In the NIR region, observed CD spectra (NIR-VCD) are a result of the combination and overtone vibrational modes of the O-H and C-H transitions as well as the CD spectra (NIR-ECD) derived from the d-d transitions of metallic proteins and metal complexes. NIR-ECD spectra of the metal protein and metal complexes are known to be sensitive to quite detailed structures including the conformation of metal ligands as well as the configuration around the central metal, which is used for structural analysis of these molecules. For NIR-VCD, theories and calculation methods to interpret the NIR-VCD spectra have been introduced, and comparison analysis with many of the spectra has been accomplished. The J-1500 CD spectrometer can be used for data collection in the NIR region (up to 1600cm-1) by replacing the detector and light source. In this configuration the instrument can be used for NIR-VCD measurement of the vibration transitions and NIR-ECD measurements of molecules including metal complexes complexes, as well as ECD spectra spectra in the UV region, primarily primarily used for measurement measurement of biological biological samples. Examples of the NIR-CD spectra of a nickel tartrate solution and limonene by the J-1500 CD.