Analysis of antiflatulents by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

Medicines contain various active ingredients and for analyzing such ingredients simultaneously, gradient elution method is useful, requiring high sensitive detection method. Therefore, for analyzing medicines in gradient mode it is quite useful to use Evaporative Light Scattering Detector (ELSD) , because it can detect almost all the compounds except volatile components with high sensitively and stable baseline.

In this report, some ingredients contained in antiflatulent were analyzed by using ELSD and PDA detector, such as stearic acid that is used as lubricants for forming tablets, ursodeoxycholic acid that is effective to improve digestion and absorption, acrinol that is used as disinfectant and berberine that has antibacterial, anti-inflammatory and gastric mucosal protection effects.