Analysis of sugar alcohols by semi-micro HPLC

Semi-micro HPLC analysis offers a significant advantage compared with conventional separations in reducing separation time and cutting cost by reducing the consumption of mobile phase. However, HPLC systems designed for conventional separations often do not provide good enough peak shape or separation due to peak diffusion in the non-optimized flow path. When using a semi-micro column an optimized RHPLC system is recommended.

This analysis report compares the measurement of sugar alcohols using a conventional refractive index detector with a detector optimized for semi-micro HPLC.