High throughput CD spectral measurement by J-1500-PAL system

Application to biomedicines, evaluation of pH dependency of human serum albumin structure

R&D of biomedicines utilizing active ingredient derived from protein is increasing day by day. However, such biomedicines are more sensitive against environmental change such as temperature, pH, and salt concentration than those of ordinary pharmaceuticals produced from low molecular compounds. And such sensitivity will be the possible cause of deactivation of biomedicines in production process. The protein structure and its activity are related closely, while CD measurement can easily provide the information regarding the change of protein structure in small amount of sample. Therefore, the CD measurement is widely used in the quality control of biomedicines including proteins. For such pharmaceutical lab, a fully automated high throughput CD spectral measurement system has been developed by Jasco in order to meet the demand for a great number of sample analyses in the short period. This system consists of model J-1500 CD spectrometer and a liquid handler, CTC PAL enabling the automation automation of sample pretreatment pretreatment, injection injection and washing washing.

In this report, this automated system has been applied to the evaluation of pH dependency of human serum albumin (HAS) structure.