CD measurement of microscale sample by J-1500 CD spectrometer

Measurement of hemoglobin and DNA derived from bovine thymus

CD measurement method is an essential tool for structural analysis of biological molecule such as protein or nucleic acid. In case of using the cell with the optical pathlength of 1mm in CD measurement, it is possible to measure the microscale sample as low as 400 ul in volume and 0.1 mg/ml in concentration, however for the precious samples there may be the case that it is difficult to get even such amount of samples.

J-1500 CD spectrometer enables the measurement of CD spectra with high accuracy and high S/N ratio of even microscale sample using the micro cell with small acceptance surface, due to the improved optical system for higher throughput and the electrical system using the latest digital lock-in detection.

In this application data, CD measurement of hemoglobin and DNA derived from bovine thymus was implemented using micro sampling disk for microscale sample and J-1500 CD spectrometer. The measurement was performed with sample volume of 3 ul, and secondary structural analysis was perf d orme for CD spectra of hemoglobin.