Extraction of synthetic cannabinoids from hydrolyzed urine using ISOLUTE SLE+ prior to GC-MS analysis

Resource type:
Application note
Format:
pdf
Date of creation:
24 October 2017
Library code:
11332
ISOLUTE Supported Liquid Extraction (SLE+) plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.
This application note describes the extraction of a range of synthetic cannabinoids and metabolites from hydrolyzed human urine prior to GC-MS. Effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 400 uL or 1 mL are described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <10% for all analytes.