Extraction of synthetic cannabinoids (SPICE) from oral fluid using ISOLUTE SLE+ prior to GC-MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE), providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of a range of synthetic cannabinoids and metabolites from oral fluid collected using the Quantisal oral fluid collection device prior to GC-MS analysis. An effective and efficient ISOLUTE SLE+ protocol has been developed that is optimized for loading volumes of either 400 uL or 1 mL of matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs <10% for all analytes.