Extraction of cocaine and metabolites from oral fluid using ISOLUTE SLE+ prior to GC-MS analysis

ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation.

This application note describes the extraction of cocaine and a range of cocaine metabolites from spiked oral fluid collected with the Quantisal oral collection device prior to GC-MS. Effective and efficient ISOLUTE SLE+ protocols optimized for loading volumes of either 400 uL or 1 mL are described. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs of <10% for all analytes.